This kit can rapidly extract total RNA from ≤20 mg animal soft tissue (liver, spleen, kidney, brain, etc.), ≤1×107 cultured cells. The kit is based on silica gel column purification technology, no need to use DTT and β-mercaptoethanol during the extraction process, and the entire extraction process can be completed within 30 minutes. The kit combines DNA filtration technology, which can efficiently filter and remove genomic DNA. The extracted total RNA has high purity and is free from protein and other impurities. It can be used for RT-PCR, Northern Blot, Poly A purification, RNase protection analysis and in vitro translation, etc. experiment.